Isolation, Characterization and Topographical Relationships of Pigment-protein Complexes from Membranes of Rhodomicrobium vannielii

Autor:	C. S. Dow, D. J. Kelly
Rok vydání:	1985
Předmět:	Gel electrophoresis Chromatography Cytochrome Protein subunit Biology biology.organism_classification Microbiology Rhodomicrobium vannielii chemistry.chemical_compound Membrane chemistry Biochemistry biology.protein Bacteriochlorophyll Polyacrylamide gel electrophoresis Rhodospirillaceae
Zdroj:	Microbiology. 131:2941-2952
ISSN:	1465-2080 1350-0872
DOI:	10.1099/00221287-131-11-2941
Popis:	Summary: Pigment-protein complexes from Rhodomicrobium vannielii were prepared by detergent solubilization of intra-cytoplasmic membranes followed by gel electrophoresis or sucrose gradient centrifugation. These procedures gave rise to two native pigmented complexes. The major one (designated B800-865) was associated with two polypeptides of Mr 11000 and 13000 and was identified with the ‘accessory’ light-harvesting complex II found in other members of the Rhodospirillaceae. The minor complex (designated B885-RC) contained both reaction centre and light-harvesting bacteriochlorophyll. Detergent fractionation and reversible chemical cross-linking, followed by two-dimensional polyacrylamide gel electrophoresis, indicated a specific relationship between a membrane-bound cytochrome c-553 and the Mr 31000 subunit of the reaction centre.
Databáze:	OpenAIRE
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