Isolation and Molecular Identification ofVibrio natriegensfrom DiseasedPortunus trituberculatusin China
Autor: | Ke-Ran Bi, Xiaojun Zhang, Huan Gao, Binlun Yan, Jingjing Sun, Xiaojian Gao |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
biology 030106 microbiology Virulence 04 agricultural and veterinary sciences Aquatic Science Portunus trituberculatus Vibrio natriegens 16S ribosomal RNA biology.organism_classification MreB Vibrio Microbiology Housekeeping gene 03 medical and health sciences 040102 fisheries biology.protein 0401 agriculture forestry and fisheries FtsZ Agronomy and Crop Science |
Zdroj: | Journal of the World Aquaculture Society. 47:854-861 |
ISSN: | 0893-8849 |
Popis: | In July 2010, heavy mortalities were seen in cultured swimming crabs, Portunus trituberculatus, from Jiangsu Province in China. Two isolates, XA1 and XA2, were isolated from cardiac muscle and somatic muscle of the moribund crabs on ZoBell's marine 2216E agar and were confirmed as the causative agent for the mass mortalities seen on that farm. The morphological and physiological characteristics as well as the biochemical tests of the two isolates were identical and were similar to Vibrio natriegens. Both were gram-negative, oxidase positive, produced acid when fermenting glucose, and failed to grow in the media containing 1% NaCl. Furthermore, the sequencing of the 16S rRNA, gapA, ftsZ, mreB, and topA genes revealed that the two isolates were identical and had a 0.99 homology coefficient with V. natriegens. The Neighbor-Joining tree based on the concatenated sequences of four housekeeping genes (gapA, ftsZ, mreB, and topA) strongly supported that XA1 was most closely related to V. natriegens within the Vibrio harveyi–related clade. In addition, pathogenicity testing using immersion challenge confirmed that strain XA1 was virulent to juvenile crabs, and the LD50 value was 1.09 × 106. This is the first report on V. natriegens as a virulent pathogen for Portunus trituberculatus. |
Databáze: | OpenAIRE |
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