Performance ofLuffa cylindricaas an immobilization matrix for the biotransformation of cholesterol byMycobacteriumspecies

Autor:	Anna Boulanger, Samuel Fouchard, Pierre Llopiz, Hamid Bou Saab, Serge Neunlist
Rok vydání:	2010
Předmět:	Matrix (chemical analysis) Adsorption Biochemistry Biotransformation Chemistry Side chain Substrate (chemistry) Solubility Cleavage (embryo) Incubation Catalysis Biotechnology
Zdroj:	Biocatalysis and Biotransformation. 28:387-394
ISSN:	1029-2446 1024-2422
DOI:	10.3109/10242422.2010.537326
Popis:	The microbial cleavage of the side chain of cholesterol is a slow process due to the low solubility of the substrate in aqueous media (< 1 μM). Cell immobilization has been shown to be an efficient technology for enhancing the yield of cholesterol biotransformation. In these experiments, living cells of Mycobacterium sp. DSM 2966 and Mycobacterium sp. DSM 2967 were immobilized by passive adsorption on different types of solid carriers. As compared to the control and other solid supports, Luffa cylindrica resulted in a 3–4-fold increase of the specific side chain cleavage activity after 7 days of incubation. Luffa cylindrica had no significant negative influence on cell growth. Furthermore, it is a natural, inexpensive, non-toxic and mechanically strong material and therefore suitable for follow-up experiments.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::67b813828c2ea623b54f128086890eb8 https://doi.org/10.3109/10242422.2010.537326 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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