New insight into the mechanism of base propenal formation during bleomycin-mediated DNA degradation

Autor:	S. H. Wu, G. W. Ashley, Glenn H. McGall, John W. Kozarich, L. Rabow, JoAnne Stubbe
Rok vydání:	1992
Předmět:	biology Chemistry Stereochemistry Acrolein General Chemistry Biochemistry Catalysis Cofactor chemistry.chemical_compound Colloid and Surface Chemistry Mechanism of action Deoxyribose medicine biology.protein Moiety Carboxylate medicine.symptom DNA Glycolic acid
Zdroj:	Journal of the American Chemical Society. 114:4958-4967
ISSN:	1520-5126 0002-7863
DOI:	10.1021/ja00039a002
Popis:	The mechanism of interaction of the antitumor antibiotic bleomycin (BLM) and cofactors with DNA to produce base propenal and 3'-phosphoglycolate and 5'-phosphate ends has been investigated. Analytical methods have been developed that allow the isolation of glycolic acid from the modified DNA without washout of isotopic labels. Results using 18 O 2 , H 2 18 O, and H 2 18 O 2 under a variety of conditions show that 1 mol of 18 O is found per mole of glycolate in the carboxylate group and is derived from the 18 O 2 not used in the formation of activated BLM. The second oxygen in the carboxylate of glycolate is derived from the 4-oxygen in the deoxyribose moiety from which it is generated
Databáze:	OpenAIRE
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