Thiopyrimidine derivatives induce cytotoxicity, cell cycle arrest and oxidative stress in breast cancer 3D-spheroids
| Autor: | Renata Trentin Perdomo, Dênis Pires de Lima, Eduardo Benedetti Parisotto, Simone Schneider Weber, Thatikonda Narendar Reddy, Ana Tereza Gomes Guerrero, Claudia Stutz Zubieta, Maria de Fatima Cepa Matos, Giovana Bicudo Gomes |
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| Rok vydání: | 2020 |
| Předmět: |
Cell cycle checkpoint
Chemistry General Chemical Engineering Cell 02 engineering and technology General Chemistry Cell cycle 010402 general chemistry 021001 nanoscience & nanotechnology medicine.disease medicine.disease_cause 01 natural sciences Biochemistry Industrial and Manufacturing Engineering 0104 chemical sciences medicine.anatomical_structure Breast cancer Materials Chemistry Cancer research medicine Cytotoxic T cell Viability assay 0210 nano-technology Cytotoxicity Oxidative stress |
| Zdroj: | Chemical Papers. 75:1211-1220 |
| ISSN: | 1336-9075 2585-7290 |
| DOI: | 10.1007/s11696-020-01383-z |
| Popis: | 3D-cultured breast cancer cells had shown relative drug resistance as compared to 2D-cultured cells, and it has been suggested as a new in vitro cell-based drug-testing platform to look for novel antitumor molecule. Given the complexity of in vivo tumors and relative drug resistance, we evaluated a promising approach of cell cancer culture looking for potential antitumor molecules. In this study, we have proposed to investigate the 3D tumor breast cancer spheroid response to two thiopyrimidine derivatives (BUC and PEC) previously tested on monolayer culture. The evaluated compounds were synthetized from cashew nutshell liquid (CNSL). Cytotoxic, antiproliferative and pro-apoptotic properties were determined in the 2D-cultured cells. Cell viability, cell cycle analyses and oxidative stress markers of 3D-spheroids were determined. Results demonstrate the cytotoxic and antiproliferative effects of these thiopyrimidine derivatives. Additionally, BUC and PEC treatment on breast cancer 3D-spheroids induced oxidative stress and cell cycle arrest in the G1 phase. Both the tested compounds induced cytotoxicity, antiproliferative effects, oxidative stress and cell cycle arrest in breast cancer 3D-spheroids and could be considered for antitumor potential in further study as treatment strategy. |
| Databáze: | OpenAIRE |
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