Screening Method for Detection of Drugs of Abuse in Human Urine

Autor:	John Beattie, Mei Lee Lowe, Norman Weissman, James A. Demetriou
Rok vydání:	1971
Předmět:	Detection limit Drugs of abuse chemistry.chemical_compound Chromatography chemistry Elution Silica gel Biochemistry (medical) Clinical Biochemistry Screening method Urine Amberlite Ultraviolet radiation
Zdroj:	Clinical Chemistry. 17:875-881
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/17.9.875
Popis:	A simple, convenient method is described for the detection in human urine of three major classes of drugs of abuse: amphetamines, barbiturates, and alkaloids. The drugs are adsorbed from urine by a column of non-ionic resin (Amberlite XAD-2), eluted with methanol, and chromatographed on a thin-layer silica gel plate. The drugs are made visible and identified by a series of procedures involving exposure to ultraviolet radiation, heat treatment, sequential spraying with group-specific reagents, and measurement of Rf values. Detection limits for 5-ml urine specimens are: 0.4 µg per ml for amphetamines and barbiturates, and 0.8 g per ml for alkaloids. The test requires 6 h to perform. One technologist can process 50 specimens per day.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::67a27fd48747152a964bddc0810e8470 https://doi.org/10.1093/clinchem/17.9.875 Zobrazit plný text záznamu