Illumina high-throughput sequencing and comparative analysis of bacterial communities in cherry orchard soil
| Autor: | Sijun Qin, Ze-yuan Yang, Deguo Lyu, Jingyun Li, Lingzhi Liu |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Rhizosphere biology Health Toxicology and Mutagenesis Bulk soil Soil classification biology.organism_classification complex mixtures Pollution Soil quality Actinobacteria 03 medical and health sciences 030104 developmental biology Botany Environmental Chemistry Proteobacteria Betaproteobacteria Acidobacteria |
| Zdroj: | Toxicological & Environmental Chemistry. 98:462-478 |
| ISSN: | 1029-0486 0277-2248 |
| Popis: | Microbial diversity in the rhizosphere is affected by soil physical and chemical properties, which may be determined by plant species and growth stage. Exploring the bacterial communities in cherry rhizospheres may help in monitoring and conservation of soil quality. The aim of this study was to characterize the bacterial community structure between bulk soil and rhizosphere, using Illumina pyrosequencing of bacterial 16S rRNA genes, to determine the effect of soil types and seasons on bacterial diversity in cherry orchard. Sequences of 199,615 (30,162–35,399/sample) genes were obtained. Rhizosphere communities shared features with the surrounding bulk soil, such as predominance of Proteobacteria, Acidobacteria, and Actinobacteria. The relative abundance of certain bacterial lineages differed: Actinobacteria, Alphaproteobacteria, and Betaproteobacteria were more abundant in the rhizosphere than in bulk soil. At the genus level, Burkholderia, Rhodococcus, Streptomyces, and Varivorax were more abund... |
| Databáze: | OpenAIRE |
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