Three-dimensional Arrangement of Short DNA Oligonucleotides at Surfaces via the Synthesis of DNA-branched Polyacrylamide Brushes by SI-ATRP
| Autor: | Olivier Y.F. Henry, Josep Luis Acero Sanchez, Ciara K. O'Sullivan, Sinead Kirwan, Ahmed D. Mehdi |
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| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Materials science Polymers and Plastics Atom-transfer radical-polymerization Oligonucleotide Aptamer Organic Chemistry Polyacrylamide 02 engineering and technology Polymer 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences chemistry.chemical_compound Monomer chemistry Polymer chemistry Materials Chemistry Nucleic acid 0210 nano-technology DNA |
| Zdroj: | Macromolecular Rapid Communications. 32:1405-1410 |
| ISSN: | 1022-1336 |
| DOI: | 10.1002/marc.201100317 |
| Popis: | Short DNA oligonucleotide branches are incorporated into acrylamide brushes via surface initiated atom transfer radical polymerization in an attempt to increase DNA surface density by building three-dimensional molecular architectures. ATR-FTIR as well as hybridization studies followed by SPR confirm the incorporation of the DNA sequences into the polymer backbone. MALDI-TOF analysis further suggests that six acrylamide monomer units are typically separating DNA branches present on a single brushes approximately 26 units long. This new approach offers a promising alternative to SAM-based nucleic acid and aptamer sensors and could enable the realization of more complex soft materials of controlled architecture capable of both recognition and signaling by including additional optically or electrochemically active moieties. |
| Databáze: | OpenAIRE |
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