Embryonic Fibroblast-Conditioned Medium Enhances Viability and Proliferation of Chick Circulating Primordial Germ Cells (cPGCs) in Suspension Culture

Autor:	Koichiro Hashimoto, Yoshinori Tamaki, Koichiro Kano, Takaharu Kawashima, Yasuyuki Kannan
Rok vydání:	2002
Předmět:	Embryo Biology Embryonic stem cell Quail In vitro Andrology medicine.anatomical_structure biology.animal Immunology medicine Animal Science and Zoology Germ Fibroblast Mitosis Fetal bovine serum
Zdroj:	Journal of Reproduction and Development. 48:143-150
ISSN:	1348-4400 0916-8818
Popis:	Primordial germ cells circulating in the embryonic vascular system (cPGCs) were isolated from 2.5-day-old chick embryos and cultured individually in suspension for 5 days in embryonic stem-cell medium (ESM) adjusted to pH 8.0 supplemented with fetal bovine serum. Most cells died within 5 days, during which time only a few cells (16-23%) underwent mitosis once, and only a very few cells (less than 3%) twice. The use of conditioned medium (CM) derived from embryonic fibroblasts enhanced the viability and proliferation of the cPGCs, resulting in survival of up to one-third of the cells for 5 days. A fraction of the cells (3-5%) even divided 3 times. The in vitro cultured cPGCs maintain the capacity to migrate into the gonadal area. The chick cPGCs cultured in vitro for up to 5 days in the CM produced germ-line chimeras upon injection into the bloodstream of 2-day-old quail embryos.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6785404fe3440b14415fca561670b345 https://doi.org/10.1262/jrd.48.143 Zobrazit plný text záznamu