Identification of β-chain of FoF1-ATPase in apoptotic cell population induced byMicroplitis bicoloratusbracovirus and its role in the development ofSpodoptera litura
| Autor: | Shan You, Sha Xu, Yang Yang, Kou Tianchao, Shu-Mei Dong, Ming Li, Ji-Hui Cui, Kai-Jun Luo, Yue-Tong Liu, Dong-Shuai Yu, Wei Zhang, Xue-Wen Zhang, Zunyu Pang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
food.ingredient Physiology Cell Population Spodoptera litura Spodoptera Biochemistry Transcriptome 03 medical and health sciences food RNA interference Botany medicine education education.field_of_study 030102 biochemistry & molecular biology biology General Medicine biology.organism_classification Cell biology 030104 developmental biology medicine.anatomical_structure Insect Science Ectopic expression Bracovirus |
| Zdroj: | Archives of Insect Biochemistry and Physiology. 95:e21389 |
| ISSN: | 0739-4462 |
| Popis: | Two physiological changes of Spodoptera litura parasitized by Microplitis bicoloratus are hemocyte-apoptosis and retarded immature development. β-Chain of FoF1-ATPase was found from a S. litura transcriptome. It belongs to a conserved P-loop NTPase superfamily, descending from a common ancestor of Lepidopteran clade. However, the characterization of β-chain of ATPase in apoptotic cells and its involvement in development remain unknown. Here, the ectopic expression and endogenous FoF1-ATPase β-chain occurred on S. litura cell membrane: in vivo, at the late stage of apoptotic hemocyte, endogenous FoF1-ATPase β-chain was stably expressed during M. bicoloratus larva development from 4 to 7 days post-parasitization; in vitro, at an early stage of pre-apoptotic Spli221 cells by infecting with M. bicoloratus bracovirus particles, the proteins were speedily recover expression. Furthermore, endogenous FoF1-ATPase β-chain was localized on the apoptotic cell membrane. RNA interference (RNAi) of FoF1-ATPase β-chain led to significantly decreased head capsule width. This suggested that FoF1-ATPase β-chain positively regulated the development of S. litura. The RNAi effect on the head capsule width was enhanced with parasitism. Our research found that FoF1-ATPase β-chain was expressed and localized on the cell membrane in the apoptotic cells, and involved in the development of S. litura. |
| Databáze: | OpenAIRE |
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