Improved Antitumor Outcomes for Colon Cancer Using Nanomicelles Loaded with the Novel Antitumor Agent LA67
| Autor: | Xue-Zhe Yin, Wei Huang, Dong-Ming Zhang, Yingda Zang, Mingji Jin, Mingfeng Han, Zhonggao Gao, Chuang-Jun Li, Minhu Cui |
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| Rok vydání: | 2020 |
| Předmět: |
Biophysics
Pharmaceutical Science Bioengineering 02 engineering and technology Pharmacology 010402 general chemistry 01 natural sciences Metastasis Biomaterials Neovascularization chemistry.chemical_compound In vivo Drug Discovery medicine Chemistry Cell growth Organic Chemistry General Medicine Triptolide 021001 nanoscience & nanotechnology medicine.disease In vitro 0104 chemical sciences Apoptosis Cancer cell medicine.symptom 0210 nano-technology |
| Zdroj: | International Journal of Nanomedicine. 15:3563-3576 |
| ISSN: | 1178-2013 |
| DOI: | 10.2147/ijn.s241577 |
| Popis: | Background LA67 is a derivative of triptolide that exhibits strong antitumor activity. This derivative has a better safety profile than triptolide, but is limited by poor aqueous solubility. Aim and methods To improve solubility and further increase therapeutic efficacy, we prepared LA67-loaded polymeric micelles (LA67-PMs) using a film hydration method. The physicochemical properties of LA67-PMs were investigated, and the antitumor activity of this formulation against Colon26 (C26) cancer cell line was evaluated in vitro and in vivo with LA67 as a control. Results Polymeric micelles containing LA67 had a particle size of 17.88 nm and a drug entrapment efficiency of 94.84%. This formulation dispersed completely in aqueous solution and exhibited slow, sustained release of LA67. Cellular uptake assay showed that LA67-PMs delivered LA67 to cancer cells with greater efficiency than free LA67, which resulted in increased LA67 accumulation in cancer cells. Cell counting kit 8 (CCK-8) assay showed that blank polymeric micelles (PMs) exhibited low toxicity and LA67-PMs exerted pronounced anti-proliferation effects against C26 cells. Furthermore, LA67-PMs induced apoptosis and repressed migration more effectively than free LA67. In vivo evaluation of antitumor activity showed that LA67-PMs inhibited tumor growth and distant organ metastasis to a greater extent than LA67, which resulted in improved survival rate. The potential mechanisms of these effects may have been induction of apoptosis, inhibition of cell proliferation, and neovascularization. Conclusion Our study showed that LA67-PMs may be a promising formulation for treatment of colon cancer. |
| Databáze: | OpenAIRE |
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