Stereochemical course of phospho group transfer by human prostatic acid phosphatase

Autor:	R. L. Van Etten, Mohan S. Saini, Jeremy R. Knowles, Stephen L. Buchwald
Rok vydání:	1984
Předmět:	chemistry.chemical_classification Stereochemistry Phosphatase Stereoisomerism Cell Biology Nuclear magnetic resonance spectroscopy Phosphate Biochemistry Hydrolysis chemistry.chemical_compound Enzyme chemistry Prostatic acid phosphatase Covalent bond Molecular Biology
Zdroj:	Journal of Biological Chemistry. 259:2208-2213
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(17)43339-4
Popis:	The stereochemical course of the phospho transfer catalyzed by homogeneous human prostatic acid phosphatase was investigated using 31P nuclear magnetic resonance spectroscopy. Transphosphorylation from phenyl-(R)-[15O, 17O, 18O]phosphate to (S)-propane-1,2-diol occurs with overall retention of configuration at phosphorus. This stereochemical result is consistent with the interpretation that the hydrolysis of substrates by this enzyme proceeds by way of a covalent phosphoenzyme intermediate. Conditions for optimizing phospho transfer by this and related acid phosphatases have also been explored.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6770b234bbe3b26c67840ef2351bcab5 https://doi.org/10.1016/s0021-9258(17)43339-4 Zobrazit plný text záznamu