| Popis: |
The synthesis of [14C] labelled Sulodexide is reported. Sulodexide is a sulphated polysaccharide of the class of glycosaminoglycan, containing a heparin like fraction (70%), dermatan sulphate (20%) and other minor fractions. The heparin-like fraction, suitably isolated from other components, was partially and selectively N-desulphated, thus making few percent unit -NH2 groups available for the labelling with [14C]-acetic anhydride (specific activity 0.15 μCi/mg). Due to the small extent of modification, the sulphate to carboxylate group ratio remained practically unchanged on the heparin-like fraction. Sulodexide was reconstituted adding to the labelled fraction the suitable amount of the other components; the chemical and biological properties of the final labelled Sulodexide were undistinguishable from those of the starting material. |
Plný text