Mouse Dual Ig Domain Containing Cell Adhesion Molecule Protein Expression and Purification Using the Baculovirus Expression Vector System

Autor:	Seung-Won Park, Gwang-gill Lee, Seok-Woo Kang, Tae-Won Goo, Seong Ryul Kim, Ji-Hyun Choi
Rok vydání:	2010
Předmět:	Signal peptide chemistry.chemical_classification Cell adhesion molecule Immunology Sf9 Immunoglobulin domain Biology Microbiology Molecular biology Transmembrane protein Amino acid law.invention chemistry Cytoplasm law Virology Recombinant DNA
Zdroj:	Journal of Bacteriology and Virology. 40:123
ISSN:	1598-2467
DOI:	10.4167/jbv.2010.40.3.123
Popis:	A baculovirus expression vector system (BEVS) is used routinely to produce recombinant proteins in the milligram scale. Dual Ig domain containing cell adhesion molecule (DICAM) belongs to the type I class of transmembrane proteins. It consists of a signal peptide, two V-type Ig domains in the extracellular region, and a short cytoplasmic tail of 442 amino acids. To purify the recombinant DICAM protein from cells overexpressing the mouse full-length DICAM gene, recombinant baculovirus is infected and recovered in the Sf9 cells. As a result, mouse DICAM protein was efficiently expressed and extracted from the insect cells using the BEVS. This recombinant protein can be used in further studies for functional test of DICAM protein in the cells.
Databáze:	OpenAIRE
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