Comparative study of fluorescence and surface-enhanced Raman scattering with magnetic microparticle-based assay for target bacterial DNA detection
| Autor: | Min Ji Hwang, Ah Seong Jang, Dong Kwon Lim |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
02 engineering and technology
010402 general chemistry medicine.disease_cause 01 natural sciences DNA sequencing Enterococcus faecalis symbols.namesake chemistry.chemical_compound Materials Chemistry medicine Electrical and Electronic Engineering Microparticle Instrumentation Escherichia coli biology Chemistry Metals and Alloys 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Nucleic acid symbols Biophysics 0210 nano-technology Raman scattering DNA |
| Zdroj: | Sensors and Actuators B: Chemical. 329:129134 |
| ISSN: | 0925-4005 |
| Popis: | Magnetic microparticles (MMPs) have been extensively utilized in various assays as protein biomarkers or nucleic acid targets using a magnetic field owing to the diverse surface chemistry and ease of separation. In this study, we designed a simple MMP-based assay for target bacterial DNA with two different optical signals: fluorescence and Raman scattering. The sensitivity and multiplexing capability of both the signals were rigorously compared under optimized assay conditions. The MMP-based assay enabled the detection of multiple target bacterial DNAs with high reliability, sensitivity, and multiplexing capability, as demonstrated by the designed target DNA sequences from Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus. The surface-enhanced Raman scattering (SERS)-based detection of the Escherichia coli genome DNA exhibited better sensitivity (∼30 fM) than that of the fluorescence method. Hence, the MMP-based assay in this scheme is a promising method of detecting bacteria. Further, the results of the systematic comparison between fluorescence and surface-enhanced Raman scattering will be beneficial for future designs of assay protocols with optical signals. |
| Databáze: | OpenAIRE |
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