Ultrastructural observations on hemocytopoiesis in embryos of the horseshoe crab, Tachypleus tridentatus
| Autor: | Ping Liang, Ting-Kai Cheng, Yi-qin Wu, Wei-hong Wu |
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| Rok vydání: | 1990 |
| Předmět: | |
| Zdroj: | Proceedings, annual meeting, Electron Microscopy Society of America. 48:506-507 |
| ISSN: | 2690-1315 0424-8201 |
| DOI: | 10.1017/s042482010016008x |
| Popis: | The main type of horseshoe crab hemocytes is amebocyte (granulocyte), whose lysate is a very sensitive indicator of endotoxin, and has been used widely in the medical and pharmaceutical fields. The origin, site of production and course of development of amebocyte haven’t been solved yet. It is still in dispute if there is another cell type in the circulating hemolymph. We have studied the fine structure of amebocytes from adult Tachypleus tridentatus, and found a small number of immature and maturing amebocytes, a few being in mitosis, and considered these findings to be full proof that some amebocytes could proliferate and mature during circulation. This paper represents a continuation of our previous work, and reports the observations on hemocytopoiesis in embryos of Tachypleus tridentatus.The embryos were obtained by artificial insemination and incubation. The specimens for light microscopic studies consisted of 17 embryos 13-21 days old, 3 embryos and 3 trilobite larvae shortly before and after hatching, while those for electron microscopy included 6 embryos of 13-19 days, 1 late embryo and 1 trilobite larva. All specimens were processed in a routine manner. |
| Databáze: | OpenAIRE |
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