Amblyomma sculptum: genetic diversity and rickettsias in the Brazilian Cerrado biome
| Autor: | S. V. de Oliveira, Marinete Amorim, Rebecca Leal Caetano, Karla Bitencourth, Carolina M. Voloch, Gilberto Salles Gazeta |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030231 tropical medicine Population Zoology Tick Nucleotide diversity 03 medical and health sciences 0302 clinical medicine parasitic diseases medicine education Ecology Evolution Behavior and Systematics Tick-borne disease Genetic diversity education.field_of_study General Veterinary biology bacterial infections and mycoses medicine.disease biology.organism_classification Rickettsia felis Virology Spotted fever 030104 developmental biology Rickettsia Insect Science bacteria Parasitology |
| Zdroj: | Medical and Veterinary Entomology. 31:427-437 |
| ISSN: | 0269-283X |
| Popis: | Amblyomma sculptum (Ixodida: Ixodidae) Berlese, 1888 is the most important tick vector in Brazil, transmitting the bioagent of the most severe form of spotted fever (SF) in part of the Cerrado (in the states of Minas Gerais and Sao Paulo). In another part of the Cerrado (Central-West region of Brazil), a milder form of SF has been recorded. However, neither the rickettsia nor the vector involved have been characterized. The aim of the current study was to analyse genetic variation and the presence of rickettsia in A. sculptum in Cerrado, from silent areas and with the milder form of SF. Samples were subjected to DNA extraction, amplification and sequencing of 12S rDNA, cytochrome oxidase subunit II and D-loop mitochondrial genes (for tick population analyses), and gltA, htrA, ompA and gene D (sca4) genes for rickettsia researches. Exclusive haplotypes with low frequencies, high haplotype diversity and low nucleotide diversity, star-shaped networks and significant results in neutrality tests indicate A. sculptum population expansions in some areas. Rickettsia amblyommatis, Candidatus Rickettsia andeanae and Rickettsia felis were detected. The A. sculptum diversity is not geographically, or biome delimited, pointing to a different potential in vector capacity, possibly associated with differing tick genetic profiles. |
| Databáze: | OpenAIRE |
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