Influence of polymerization parameters and entrapment in poly(hydroxyethyl methacrylate) on activity and stability of GOD
| Autor: | Peter Abel, Anne Riedel, Birgit Schulz |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
chemistry.chemical_classification
biology Process Chemistry and Technology Radical technology industry and agriculture Bioengineering macromolecular substances Polymer (Hydroxyethyl)methacrylate Biochemistry humanities Catalysis chemistry.chemical_compound Enzyme Monomer chemistry Polymerization Polymer chemistry biology.protein Glucose oxidase Biosensor |
| Zdroj: | Journal of Molecular Catalysis B: Enzymatic. 7:85-91 |
| ISSN: | 1381-1177 |
| Popis: | The functional stability of biosensors used in vivo seems, most probably, to be limited by enzyme instability. Therefore, investigations have been carried out on enzyme stabilization by immobilization using poly(hydroxyethyl methacrylate) (pHEMA). On one hand, there are several factors connected to polymerization of the monomer hydroxyethyl methacrylate (HEMA) able to influence the activity of enzymes during their entrapment in the polymer. The factors we investigated are: the concentration of the monomer, different temperatures (50–65°C), ultraviolet radiation, and the concentration of radicals generated by different initiator concentrations. Glucose oxidase (GOD) was used as a model enzyme. It was found that the most important GOD inactivating factors are the monomer itself and temperatures higher than 50°C. On the other hand, despite of these influences a long term stable immobilization of GOD by entrapment in pHEMA could be realized. |
| Databáze: | OpenAIRE |
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