Differential uptake of gold nanoparticles by 2 species of tadpole, the wood frog (Lithobates sylvaticus ) and the bullfrog (Lithobates catesbeianus )
| Autor: | Kurt Andresen, Kevin T. Lerner, Laura L. Lee, Gerardo L. F. Carfagno, Peter P. Fong, Andrea J. Sitton, Lucas B. Thompson, Taylor B. Bury |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
biology
Health Toxicology and Mutagenesis Lithobates Zoology 02 engineering and technology 010501 environmental sciences 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences Engineered nanoparticles Tadpole Anterior region Aquatic toxicology Toxicology Bullfrog Colloidal gold Environmental Chemistry sense organs 0210 nano-technology Large group 0105 earth and related environmental sciences |
| Zdroj: | Environmental Toxicology and Chemistry. 36:3351-3358 |
| ISSN: | 0730-7268 |
| DOI: | 10.1002/etc.3909 |
| Popis: | Engineered nanoparticles are aquatic contaminants of emerging concern exerting ecotoxicological effects on a wide variety of organisms. We exposed cetyltrimethylammonium bromide capped spherical gold nanoparticles to wood frog and bullfrog tadpoles with conspecifics and in combination with the other species continuously for 21 days, then measured uptake and localization of gold. Wood frog tadpoles alone and in combination with bullfrog tadpoles took up significantly more gold than bullfrogs. Bullfrog tadpoles in combination with wood frogs took up significantly more gold than controls. The rank order of weight normalized gold uptake was wood frogs in combination > wood frogs alone > bullfrogs in combination > bullfrogs alone > controls. In all gold-exposed groups of tadpoles, gold was concentrated in the anterior region compared to the posterior region of the body. The concentration of gold nanoparticles in the anterior region of wood frogs both alone and in combination with bullfrogs was significantly higher than their corresponding posterior regions. We also measured depuration time of gold in wood frogs. After 21 days in a solution of gold nanoparticles, tadpoles lost over 83% of internalized gold when placed in gold-free water for 5 days. After 10 days in gold-free water, tadpoles lost 94 % of their gold. After 15 days, gold concentrations were below the level of detection. Our finding of differential uptake between closely related species living in similar habitats with overlapping geographical distributions argues against generalizing toxicological effects of nanoparticles for a large group of organisms based upon measurements in only one species. This article is protected by copyright. All rights reserved |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text