A poloxamer-polypeptide thermosensitive hydrogel as a cell scaffold and sustained release depot
| Autor: | Li-Yu Lee, Yuan-Kai Lin, Ji-Yu Lin, Chieh-Nan Chen, I-Ming Chu, Po-Liang Lai, Sydney Peng |
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| Rok vydání: | 2016 |
| Předmět: |
Scaffold
Polymers and Plastics biology Chemistry Depot Organic Chemistry Bioengineering Nanotechnology 02 engineering and technology Poloxamer 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Micelle 0104 chemical sciences Tissue engineering Drug delivery biology.protein Copolymer Biophysics Bovine serum albumin 0210 nano-technology |
| Zdroj: | Polymer Chemistry. 7:2976-2985 |
| ISSN: | 1759-9962 1759-9954 |
| DOI: | 10.1039/c5py02067k |
| Popis: | Herein, we report the synthesis and characterization of a novel positively charged thermosensitive hydrogel prepared from poloxamer (PLX)-poly(L-alanine-lysine) (Lys-Ala-PLX-Ala-Lys) that demonstrates potential in biomedical applications including tissue engineering and drug delivery. At a dilute concentration, the copolymer self-assembled into micelles that were sensitive to temperature elevation. In the concentration range of 3–7 wt%, the aqueous copolymer solution underwent sol–gel transition near the physiological temperature and exhibited changes in its secondary structure content, as evidenced by FT-IR. Excellent viability of cells cultured within the scaffold was observed after 72 h of incubation. On the other hand, negatively charged bovine serum albumin was incorporated into the hydrogel without diminishing material integrity. Over the course of seven days, the protein underwent extended zero-order release. These findings highlight the potential of this system as a cell scaffold as well as a depot for the extended delivery of drugs and proteins, specifically those which bear a charge opposite to that of the hydrogel. |
| Databáze: | OpenAIRE |
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