Subcellular Distribution of Cadmium and Nickel in Chronically Exposed Wild Fish: Inferences Regarding Metal Detoxification Strategies and Implications for Setting Water Quality Guidelines for Dissolved Metals
| Autor: | Lisa D. Kraemer, Landis Hare, Peter G. C. Campbell, Alice Hontela, Anik Giguère |
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| Rok vydání: | 2008 |
| Předmět: |
Differential centrifugation
Perch Cadmium Health Toxicology and Mutagenesis Ecological Modeling chemistry.chemical_element Biology biology.organism_classification Pollution Metal Cytosol chemistry Bioaccumulation Environmental chemistry visual_art Toxicity visual_art.visual_art_medium Metallothionein |
| Zdroj: | Human and Ecological Risk Assessment: An International Journal. 14:290-316 |
| ISSN: | 1549-7860 1080-7039 |
| DOI: | 10.1080/10807030801935009 |
| Popis: | The objective of this study was to investigate metal detoxification in chronically exposed juvenile yellow perch (YP: Perca flavescens) and to field test the commonly assumed threshold toxicity model. Fish were collected from lakes located along a cadmium (Cd) and nickel (Ni) concentration gradient. Ambient dissolved metal concentrations were measured to evaluate exposure and total hepatic metal concentrations were determined as a measure of metal bioaccumulation. Hepatic metal partitioning among potentially metal-sensitive fractions (heat-denatured proteins, organelles) and detoxified metal fractions (metallothionein) was determined after differential centrifugation of YP liver homogenates. Major proportions of hepatic Cd were found in the heat-stable cytosolic peptides and proteins fraction (HSP; including metallothioneins), whereas Ni was mainly found in the potentially metal-sensitive heat-denaturable proteins fraction (HDP). For these chronically exposed fish there was no threshold exposure ... |
| Databáze: | OpenAIRE |
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