A Single-tube real-time RT-PCR assay for RNA detection and quantification of genetically diverse HIV including rare HIV-1 non-m groups and HIV-2
| Autor: | N. M. Gashnikova, G. M. Dymshits, M. A. Prasolova, E. S. Netesova, M. K. Ivanov |
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| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Genetic variants Human immunodeficiency virus (HIV) virus diseases RNA Biology medicine.disease_cause Microbiology Virology Molecular biology Single tube Infectious Diseases Real-time polymerase chain reaction chemistry Genotype Blood plasma Genetics medicine Nucleotide Molecular Biology |
| Zdroj: | Molecular Genetics, Microbiology and Virology. 29:179-185 |
| ISSN: | 1934-841X 0891-4168 |
| DOI: | 10.3103/s0891416814040089 |
| Popis: | The RT-PCR method with real-time fluorescence detection was used for the development of a prototype of a diagnostic kit for reliable diagnosis of genetic variants of RNA of HIV-1 groups M, N, O, and P and RNA of HIV-2 in blood plasma and serum. The kit is tolerant to nucleotide mismatches, provides a broad linear range of concentrations of HIV RNA, 100% analytical specificity, and adequate analytical sensitivity: 42 ME/mL (HIV-1 group M), 45 copies/mL (HIV-2), 92 copies/mL (HIV-1 group O), and 90 copies/mL (HIV-1 group N). The kit provided successful detection and measurement of HIV RNA concentration in all the samples of the international reference panel for HIV-1 genotypes. The results of the test correlate with the results of commercial tests. |
| Databáze: | OpenAIRE |
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