Natural resistance of BALB/c mice to herpes simplex virus-type 1 intraperitoneal infection is abrogated by a plant extract within vitro anti-herpes activity

Autor: J. D. Claus, Celia E. Coto, R A de Torres
Rok vydání: 1998
Předmět:
Zdroj: Phytotherapy Research. 12:250-254
ISSN: 1099-1573
0951-418X
DOI: 10.1002/(sici)1099-1573(199806)12:4<250::aid-ptr286>3.0.co;2-x
Popis: Adult BALB/c mice, genetically resistant to HSV-1 i.p. infection, were inoculated intraperitoneally with 1000 PFU of HSV-1 and treated by the same route with four consecutive doses of Melia azedarach L. aqueous green leaves extract (MA) each containing 80 antiviral units against HSV-1 replication in Vero cells. Mice were injected 1 day and 6 h before infection and 1 and 2 days after infection. Mortality among untreated 45 day old female and male mice was around 35% for both sexes, whereas MA treatment increased mortality to 95% in females and to 50% in males. Virus was isolated from brains of treated animals. Enhanced susceptibility to HSV-1 infection upon treatment with MA extract was also observed in male mice 25 days old, before hormonal maturation, indicating that the impairment of natural resistance to HSV-1 infection is sex-linked. Treatment with MA extracts did not interfere with established specific immune protection nor with the induction of immune response to HSV-1. Virus was recovered from peritoneal exudate cells only in MA-treated animals. The abrogation of natural resistance of inbred mice to i.p. HSV-1 infection by pretreatment and early administration of the plant extract indicates that the in vivo expression of immunomodulating activities on receptor cells interfered with its potent antiviral activity displayed in vitro against HSV-1 replication. © 1998 John Wiley & Sons, Ltd.
Databáze: OpenAIRE
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