Chromobacterium phragmitis sp. nov., isolated from estuarine marshes
| Autor: | Ashaki D. Mitchell, Michael B. Blackburn, Daniel Kuhar, Michael E. Sparks, Joseph Mowery, Robert R. Farrar, Dawn E. Gundersen-Rindal |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Phylogenetic tree Strain (biology) General Medicine Biology biology.organism_classification 16S ribosomal RNA 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences 030104 developmental biology Taxon Chromobacterium Botany Chromobacterium violaceum Ecology Evolution Behavior and Systematics Bacteria Betaproteobacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 69:2681-2686 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.003508 |
| Popis: | Thirteen isolates of Gram-stain-negative, motile, violet-pigmented bacteria were isolated from marshes along tidal portions of the Potomac and James rivers in Maryland and Virginia, USA, respectively. 16S rRNA gene sequences and fatty acid analysis revealed a high degree of relatedness among the isolates, and genomic sequencing of two isolates, IIBBL 112-1T and IIBBL 274-1 (from the Potomac and James rivers, respectively), revealed highly similar genomic sequences, with a blast-based average nucleotide identity (ANIb) of ca. 98.7 %. Phylogenetic analysis of 16S rRNA gene sequences suggested that the species most highly related to IIBBL 112-1T were Chromobacterium amazonense , Chromobacterium subtsugae and Chromobacterium sphagni . However, deletion of a 25-nucleotide sequence that may have been horizontally acquired by both IIBBL 112-1T and C. amazonense resulted in a substantially different analysis; in the latter case, the species nearest IIBBL 112-1T were Chromobacterium violaceum , Chromobacterium vaccinii and Chromobacterium piscinae . Whole-genome alignments between either IIBBL 112-1T or IIBBL 274-1 and the type strains of C. vaccinii or C. violaceum resulted in ANIb values in the range of ca. 87 %, while alignment with C. amazonense CBMAI 310T resulted in an ANIb of ca. 83 %. Collectively, these data demonstrate that IIBBL 112-1T and IIBBL 274-1 represent a new taxon within the genus Chromobacterium . We propose the name Chromobacterium phragmitis sp. nov. for this taxon; the type strain is IIBBL 112-1T (=NRRL B-67132T=JCM 31884T). |
| Databáze: | OpenAIRE |
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