Characterization of an abscisic acid carrier in suspension-cultured barley cells

Autor:	Michel R. Perras, Suzanne R. Abrams, John Balsevich
Rok vydání:	1994
Předmět:	chemistry.chemical_classification Absorption (pharmacology) Chromatography Physiology fungi food and beverages Plant Science Metabolism Biology Sesquiterpene Amino acid chemistry.chemical_compound chemistry Biochemistry Cell culture Hordeum vulgare Enantiomer Abscisic acid
Zdroj:	Journal of Experimental Botany. 45:1565-1573
ISSN:	1460-2431 0022-0957
DOI:	10.1093/jxb/45.11.1565
Popis:	The uptake of [ 3 H]-abscisic acid in barley (Hordeum vulgare L. cv. Heartland) cell cultures was found to be mediated through both non-saturable and saturable components. The kinetic parameters of the saturable component, determined at pH 4.5 and 21° C, showed a K m for natural or (+).ABA of 1.3 ± 0.7 μM and an apparent V max of 7.0 ± 2.8 nmol g −1 cells h −1 . The carrier showed a strong preference for the natural enantiomer of ABA as compared to the unnatural one. Other substances tested, e.g. amino acids, organic acids, and other growth regulators, did not appear to interfere with the carrier-mediated uptake of ABA. At low external concentrations of ABA (below 2.0 μM), the saturable component was greater than the diffusion component
Databáze:	OpenAIRE
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