Differentiation-dependent elevation of cholera toxin-stimulated intracellular cAMP production in human salivary gland adenocarcinoma cell line and its differentiated cells in culture
| Autor: | Yasuo Kasai, Hitoshi Kawamata, Masayuki Azuma, Tetsuo Yanagawa, Hideo Yoshida, Hiroki Iga, Mitsunobu Sato |
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| Rok vydání: | 1988 |
| Předmět: | |
| Zdroj: | Japanese Journal of Oral & Maxillofacial Surgery. 34:1540-1547 |
| ISSN: | 2186-1579 0021-5163 |
| DOI: | 10.5794/jjoms.34.1540 |
| Popis: | We have already reported that a neoplastic human salivary intercalated duct cell line, HSG, which has ultrastructure and biological markers specific to the intercalated duct cells of human salivary gland, can differentiate into both myoepithelial cells and acinar cells after treatment with 5-azacytidine. In addition, these differentiated cells were found to carry decreased tumorigenicity and anchorage-independent growth potential as compared to their parental HSG cells.On the other hand, it has been suggested that cyclic adenosine 3', 5'-monophosphate (cAMP) plays essential roles in controlling cellular proliferation and/or differentiation. For example, in mouse fibroblast lines including 3T3 cells, the growth potentials of cells are inversely associated with the intracellular cAMP levels. In addition, it has been demonstrated that murine erythroleukemia cells and human or murine neuroblastoma cells can be induced to differentiate in response to cAMP. Moreover, an elevation of cAMP in murine neuroblastoma cells has been reported to cause loss of tumorigenicity. Therefore, we examined the differentiation-associated enhancement of cholera toxin-mediated intracellular cAMP production in HSG cells and their differentiated cells.Consequently, the intracellular cAMP levels in response to cholera toxin were higher in the differentiated cells than in their parental HSG cells. Autoradiographic analysis found that the cholera toxin-catalyzed [32P] ADP-ribosylation of Gs protein present in cytoplasmic membranes from the differentiated cells increased as compared to untreated controls. These findings suggest that the cellular proliferation and differentiation in human salivary gland adenocarcinoma cell line HSG are assessed by the levels of cholera toxin-stimulated intracellular cAMP. |
| Databáze: | OpenAIRE |
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