Expression of hsa-miR-9 and MYC Copy Number Variation in Hereditary Diffuse Gastric Cancer
| Autor: | Kelly Cristina da Silva Oliveira, Leticia Martins Lamarão, Diego Di Felipe Avila Alcantara, Paulo Pimentel Assumpção, Amanda Braga Bona, Caroline Aquino Moreira-Nunes, Thayanne Macedo Pinheiro, Danielle Queiroz Calcagno, Fernanda Jardim Da Silva, Rommel Rodríguez Burbano |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetics Cancer Research General Medicine Oncomir Biology medicine.disease CDH1 body regions 03 medical and health sciences 030104 developmental biology Germline mutation Oncology Downregulation and upregulation Transcription (biology) embryonic structures Cancer research medicine biology.protein Copy-number variation Hereditary diffuse gastric cancer Gene |
| Zdroj: | Anticancer Research. 37:2401-2406 |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.11579 |
| Popis: | BACKGROUND/AIM Approximately, 15-50% of families affected by hereditary diffuse gastric cancer (HDGC) exhibit CDH1 germline mutations. CDH1 gene encodes E-cadherin, protein essential to the cell-cell contact of gastric epithelium. Studies have shown that hsa-miR-9 participates in this protein downregulation. Moreover, MYC is responsible for the transcription of hsa-miR-9-3. In the present study, hsa-miR-9 expression and MYC copy number variation were investigated to elucidate the hsa-miR-9 role in HDGC. PATIENTS AND METHODS Tumor samples were obtained from nine individuals with HDGC history belonging to four Brazilian families. Then, relative quantification of hsa-miR-9 expression and MYC gene copy number variation analysis were performed by real-time PCR. RESULTS In all the samples, an overexpression of hsa-miR-9 and an increased MYC copy number (≥3 copies) were observed. CONCLUSION hsa-miR-9 acts as an oncomiR in HDGC. In addition, we suggest that hsa-miR-9 acts as second event in individuals with HDGC carrying CDH1 gene germinline mutations. |
| Databáze: | OpenAIRE |
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