Impact of Antifungal Compounds on Viability and Anti- Aspergillus Activity of Human Natural Killer Cells
Autor: | Ralf Schubert, Stanislaw Schmidt, Lars Tramsen, Thomas Lehrnbecher |
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Rok vydání: | 2019 |
Předmět: |
Pharmacology
Voriconazole 0303 health sciences Posaconazole Adoptive cell transfer 030306 microbiology business.industry medicine.medical_treatment Immunotherapy Aspergillosis medicine.disease 03 medical and health sciences chemistry.chemical_compound Infectious Diseases chemistry Amphotericin B deoxycholate Amphotericin B medicine Pharmacology (medical) Caspofungin business 030304 developmental biology medicine.drug |
Zdroj: | Antimicrobial Agents and Chemotherapy. 63 |
ISSN: | 1098-6596 0066-4804 |
DOI: | 10.1128/aac.01993-18 |
Popis: | Despite the availability of new antifungal compounds, invasive aspergillosis carries high morbidity and mortality in hematopoietic stem cell transplant recipients. In vitro studies and animal models suggest that the adoptive transfer of natural killer (NK) cells might be a promising immunotherapeutic option in this setting. As it is unclear whether the viability and function of human NK cells are affected by common antifungal agents, we analyzed the interaction of various concentrations of amphotericin B deoxycholate (AmB-D), liposomal amphotericin B, caspofungin, fluconazole, voriconazole, and posaconazole with human NK cells. When adding NK cells to therapeutic concentrations of antifungal agents, a significant increase in the antifungal effect was seen for caspofungin and voriconazole, whereas NK cells significantly decreased the hyphal damage of escalated doses of AmB-D. In contrast, therapeutic concentrations of all antifungal compounds tested did not have a negative effect on proliferation, viability, and the release of soluble immunomodulatory molecules of NK cells. These data indicate that therapeutic concentrations of the antifungal agents tested do not negatively affect the functional properties of human NK cells, which is a prerequisite for further studies evaluating NK cells as antifungal immunotherapy in immunocompromised patients suffering from invasive aspergillosis. |
Databáze: | OpenAIRE |
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