A microfluidic sperm-sorting device based on rheotaxis effect
| Autor: | Waseem Asghar, Andy W.C. Lau, Afrouz Ataei |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
endocrine system
education.field_of_study Sperm sorting urogenital system 010401 analytical chemistry Population Microfluidics 02 engineering and technology Reproductive technology Biology 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Sperm Specific flow 0104 chemical sciences Electronic Optical and Magnetic Materials Cell biology Rheotaxis Materials Chemistry DNA fragmentation 0210 nano-technology education reproductive and urinary physiology |
| Zdroj: | Microfluidics and Nanofluidics. 25 |
| ISSN: | 1613-4990 1613-4982 |
| DOI: | 10.1007/s10404-021-02453-8 |
| Popis: | The ultimate challenge for assisted reproductive technologies (ARTs) is to select the most competent sperm population from a semen sample in an efficient way. In this paper, we report on an effective sperm sorting microfluidic device that exploits the rheotaxis of sperm and investigates the sperm quality sorted under various flow conditions. Rheotaxis is the ability of a sperm cell to orient itself in the direction of the flow and swim against it. A novel passively driven pumping system is reported that provides a steady flow rate while it requires no external power source. We demonstrate that sperm selected with this device at a specific flow rate have higher motility, normal morphology, and a fewer degree of DNA fragmentation compared to a control group |
| Databáze: | OpenAIRE |
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