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Thispaper studies the method of detecting antibiotic trimethoprim (TMP) in theenvironment water, sewage and tap water with high performance liquidchromatography –diode array detector (HPLC-DAD). The chromatographic conditionis confirmed that: the stationary phase is ODS, the mobile phase is acetonitrileand methanoic acid aqueous solution of 0.1%（12:88）, the flow rate is 1.5min·L-1; theinjection volume is 5μL，the chromatographic column temperature is 25°C,DAD detection wavelength is 272nm. The experiment result shows that, the linearlydependent coefficient of external standard curve is 0.996, the detection limitis 0.017 mg·L-1, adding standard recovery ranges from 92.6-140.6 ％. |
