Anticoagulant potential and total phenolic content of six species of the genus Ficus from Azad Kashmir, Pakistan
| Autor: | Aamar Mushtaq, Maqsood Ahmed, Muhammad Tariq, Imran Ali, Muhammad Shareef Masoud, Muhammad Qasim, Rehana Asghar, Sumaira Ambreen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Ficus auriculata
music.instrument biology Traditional medicine ved/biology ved/biology.organism_classification_rank.species Ficus benghalensis Pharmaceutical Science Ficus 04 agricultural and veterinary sciences 010501 environmental sciences biology.organism_classification 01 natural sciences chemistry.chemical_compound chemistry Phytochemical Ficus palmata 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Pharmacology (medical) Ficus religiosa Gallic acid Ficus semicordata music 0105 earth and related environmental sciences |
| Zdroj: | Tropical Journal of Pharmaceutical Research. 18:1245-1251 |
| ISSN: | 1596-9827 1596-5996 |
| Popis: | Purpose: To investigate the total phenolic and flavonoid contents of Ficus benghalensis, Ficus elasticaa, Ficus palmata, Ficus religiosa, Ficus semicordata and Ficus auriculata, and to determine their anticoagulant potential. Methods: Crude methanol extracts were prepared from the plant leaves, and fractionated using liquidliquid partition with n-hexane, chloroform and ethyl acetate. The total flavonoid and total phenolic contents of the extracts and their fractions were determined. The anticoagulant potential of the six Ficus species were evaluated in healthy human plasma, using activated partial thromboplastin time (APTT) and prothrombin time (PT) methods. Results: Phytochemical analysis showed the presence of considerable amounts of flavonoids ranging from 5.3 ± 0.7 to 11.8 ± 0.3 mg rutin equivalents (RE)/g, and phenolic compounds ranging from 8.0 ± 0.7 to 86.5 ± 1.5 mg gallic acid equivalents (GAE)/g in each fraction of the six species. Results from in vitro anticoagulant potential assays showed significant anticoagulant properties, with prothrombin time (PT) ranging from 17.7 ± 0.7 to 26.7 ± 2.2 s, and activated partial thromboplastin time (APTT) varying from 47.7 ± 3.3 to 72.3 ± 5.4 s. Conclusion: The results indicate that F. semicordata and F. Religiosa have higher anticoagulant potential than the other Ficus species studied. |
| Databáze: | OpenAIRE |
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