Evaluation of Anti-inflammatory, Antioxidant and Xanthine Oxidase Inhibitory Potential of N-(2-hydroxy phenyl) Acetamide

Autor: Anum Arif, Saba Majeed, null Shabana Usman Simjee, null Mariam Sarfaraz, null Sadia Suri Kashif, null Muneeza Lodhi, null Najeeb Khatian, null Aisha Aziz, Hiba Kanawal Majeed
Rok vydání: 2022
Předmět:
Zdroj: Pak-Euro Journal of Medical and Life Sciences. 5:309-318
ISSN: 2707-448X
2707-4471
DOI: 10.31580/pjmls.v5i2.2528
Popis: Nonsteroidal anti-inflammatory drugs has long been used to treat various pathological conditions like arthritis, gout, diabetes, cancer, and cardiovascular diseases. Although exploring the effective anti-inflammatory agents possessing antioxidant along with pain relieving properties is still challenging. Acetamide-containing compounds are known to possess analgesic and anti-inflammatory activity. The purpose of this study is to evaluate the anti-inflammatory, anti-oxidant, xanthine oxidase, and analgesic activity of an acetamide derivative, N-(2-Hydroxyphenyl) Acetamide. In-vitro anti-inflammatory and antioxidant activities were assessed by protein denaturation inhibition and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, respectively. Xanthine oxidase potential was also evaluated. In-vivo anti-inflammatory activity was determined by the Formalin-induced paw edema method and analgesic potential was evaluated by an acetic acid-induced writhing test. Results of in-vitro anti-inflammatory activity showed significant (p< 0.001) inhibition of protein denaturation by N-(2-Hydroxyphenyl) Acetamide at both100 and 300 µg/ml. It also exhibited high radical scavenging activity (88.16%) by DPPH at 300 µg/ml. In-vivo Formalin-induced paw edema method revealed significant (p < 0.05) inhibition of paw edema when compared with control and formalin groups. Likewise, NA-2 also significantly (p < 0.001) reduced the number of writhes in the Acetic acid-induced writhing test. Moreover, in both aforementioned in-vivo tests, NA-2 showed comparable results with Indomethacin (10 mg/kg). This study demonstrates that N-(2-Hydroxyphenyl) Acetamide possesses substantial anti-inflammatory, antioxidant, and analgesic activity.
Databáze: OpenAIRE
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