CYTOPROTECTIVE ACTIVITY OF ETHANOL FRACTION OF COLEUS AMBOINICUS LOUR. LEAVES AGAINST VERO CELLS INDUCED BY H2O2
| Autor: | Denny Satria, Urip Harahap, Masfria Masfria, Reny Haryani |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Pharmacology
Ethanol food.ingredient medicine.diagnostic_test 010405 organic chemistry Pharmaceutical Science chemistry.chemical_element Coleus 01 natural sciences Molecular biology Oxygen 0104 chemical sciences Flow cytometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food chemistry Apoptosis Bromide medicine Vero cell Pharmacology (medical) 030212 general & internal medicine Hydrogen peroxide |
| Zdroj: | Asian Journal of Pharmaceutical and Clinical Research. 11:28 |
| ISSN: | 2455-3891 0974-2441 |
| DOI: | 10.22159/ajpcr.2018.v11s1.26559 |
| Popis: | Objective: The objective of this study is to assess the cytoprotective activity of Coleus amboinicus Lour. leaves ethanol fraction (EtF) in Vero cells which induced by hydrogen peroxide (H2O2).Methods: Cytoprotective activity was determined using 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT) method, apoptosis activity was analyzed by flow cytometry, and expression of radical spesies oxygen (ROS) was observed by imunocytochemistry.Result: The EtF (100 μg/mL) was showed the highest percent viability 74.42±0.28% using MTT method. Furthermore, the EtF was increased viability of Vero cell after induced with 0.8 mM H2O2 and EtF was decreased the expression of ROS.Conclusion: Based on the description, EtF has cytoprotective activity towards Vero cells which induced by 0.8 mM H2O2 . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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