| Autor: |
Xiong Ying Miao, Li Xiong, Liang Wu Lin, Yu Wen, Xiao Feng Deng |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Advanced Materials Research. :930-938 |
| ISSN: |
1662-8985 |
| DOI: |
10.4028/www.scientific.net/amr.622-623.930 |
| Popis: |
In this study, Photosan (PS) was loaded onto hollow silica nanospheres using one-step wet chemical-based synthetic route method. Polydispersive index of the Photosan-loaded hollow Silica Nanospheres (PS-loaded NSs) is 0.121±0.01. The photobiological activity of the PS-loaded NSs was evaluated on human cholangiocarcinoma QBC939 cells. Cells were incubated with free PS or PS-loaded NSs for 2 h and then washed with phosphate-buffered saline. Culture medium was added to the wells containing the cells. Finally, the cells were exposed to red light (630 nm) with a light dose of 10J/cm2. The cellular viability was determined after 24 h of incubation. PS-loaded NSs and free PS eliminated about 96.4%±2.0% and 54.7%±1.9% of QBC939 cells, respectively. The phototoxicity was time dependent up to 2 h and concentration dependent at 1-7.5 mg/l. The cells viability decreased with the increase of the light dose in the range of 5-15 J/cm2. In conclusion, PS-loaded NSs are the release systems that promise photodynamic therapy use. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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