Isolation and Characterization of Hemopoietic Cells From Lungs of Allergic Mice
| Autor: | Danielle Joseph, B. Boris Vargaftig, Maria Ignez C Gaspar Elsas, Pedro Xavier Elsas, Elisabeth S Maximiano, Adriana Bonomo |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Pulmonary and Respiratory Medicine
Lung biology CD34 respiratory system Eosinophil Critical Care and Intensive Care Medicine respiratory tract diseases Ovalbumin Haematopoiesis medicine.anatomical_structure Granulocyte macrophage colony-stimulating factor Immunology Parenchyma medicine biology.protein Bone marrow Cardiology and Cardiovascular Medicine medicine.drug |
| Zdroj: | Chest. 123:345S-348S |
| ISSN: | 0012-3692 |
| DOI: | 10.1016/s0012-3692(15)35201-6 |
| Popis: | We developed a procedure for the isolation of hemopoietic cells from murine lung. Ovalbumin sensitization and challenge increased the numbers of functionally intact hemopoietic progenitors recovered from digested lung fragments by 80-fold to 120-fold, relative to naive controls. Eosinophil precursors, which are absent in the naive mouse lung, accumulated in the lungs of sensitized/challenged mice. Progenitors in allergic BALB/c mice were recoverable from lung parenchyma, not blood or airways, and were exclusively CD34+. Precursors isolated from allergic lung, unlike those from bone marrow, were inhibited by dexamethasone and were stimulated by prostaglandin D 2 . This directly demonstrates that sensitized/challenged lungs accumulate hemopoietic progenitors and precursors, distinct from those in bone marrow. |
| Databáze: | OpenAIRE |
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