Sharpening gene editing toolbox in Arabidopsis for plants
| Autor: | Thakku R. Ramkumar, Binod Kumar Mahto, Sangram K. Lenka, Sagar S. Arya |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Effector Classical genetics food and beverages Plant Science Computational biology biology.organism_classification 01 natural sciences Genome Zinc finger nuclease Genome engineering 03 medical and health sciences 030104 developmental biology Genome editing Arabidopsis CRISPR Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
| Zdroj: | Journal of Plant Biochemistry and Biotechnology. 29:769-784 |
| ISSN: | 0974-1275 0971-7811 |
| Popis: | Arabidopsis thaliana is considered as an indispensable model system across various disciplines of modern plant biology. The short life cycle, well developed classical genetics, small genome, and a plethora of distinguishable phenotypic features make it the most preferred plant system for researchers globally. Owing to these advantages, it is highly explored to study plant-pathogen interaction, comparative omics, evolutionary plasticity, epigenetics, and recently being used for development of precision genome editing tools for plants. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) protein system, discovered as a part of bacterial immune system is widely used as a molecular tool for targeted genome engineering. This tool superseded two well-established genome editing platforms, zinc finger nucleases and transcription activator-like effector nucleases. Here, we have discussed CRISPR/Cas based gene editing toolbox designed, sharpened and used in A. thaliana. This review focuses on pioneering innovations in CRISPR/Cas technology and subsequent application of these tools in A. thaliana which is leading to wider application of CRISPR toolbox for basic and translational research in other plants. |
| Databáze: | OpenAIRE |
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