Digital droplet LAMP as a microfluidic app on standard laboratory devices
| Autor: | Roland Zengerle, Clara Siber, Nils Paust, Simon Wadle, Felix von Stetten, Friedrich Schuler, Sebastian Hin |
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| Rok vydání: | 2016 |
| Předmět: |
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Materials science Thermal cycler Dynamic range General Chemical Engineering 010401 analytical chemistry Microfluidics General Engineering Loop-mediated isothermal amplification Microscope slide Nanotechnology 02 engineering and technology 021001 nanoscience & nanotechnology Chip 01 natural sciences 0104 chemical sciences Analytical Chemistry Standard curve 0210 nano-technology Biomedical engineering |
| Zdroj: | Analytical Methods. 8:2750-2755 |
| ISSN: | 1759-9679 1759-9660 |
| Popis: | Digital nucleic acid amplification methods are a growing research field that allows for absolute quantification of DNA making the need of standard curves redundant. However, most of the existing digital amplification systems require specialized laboratory devices and costly investments. The required disposable cartridges are device specific and not interchangeable. Here, we present digital droplet loop-mediated isothermal amplification (ddLAMP) as a microfluidic app on standard laboratory devices. ddLAMP is implemented on a disposable polymer chip (DropChip) in the format of a standard microscope slide. After off-chip DNA denaturation, the reaction mix is emulsified in the DropChip in a mini centrifuge for 6 minutes. The DropChip is transferred to an in situ thermal cycler for 1 hour of incubation. Afterwards, a fluorescence scan in a microarray scanner is performed. The DropChip allows for absolute quantification with a dynamic range of 15–1500 DNA copies per μl. Assay conditions were optimized for ddLAMP and comparison of ddPCR and ddLAMP for genomic E. coli DNA reveals very good concordance. |
| Databáze: | OpenAIRE |
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