Physical and genetic map of the Methanobacterium wolfei genome and its comparison with the updated genomic map of Methanobacterium thermoautotrophicum Marburg

Autor:	Rolf Stettler, Thomas Leisinger, Gaël Erauso
Rok vydání:	1995
Předmět:	Genetics Methanobacterium Chromosome General Medicine Biology biology.organism_classification Biochemistry Microbiology Genome Restriction fragment Restriction enzyme Gene mapping biology.protein RRNA Operon Molecular Biology Genomic organization
Zdroj:	Archives of Microbiology. 163:205-210
ISSN:	1432-072X 0302-8933
DOI:	10.1007/bf00305354
Popis:	A physical and genetic map of the chromosome of Methanobacterium wolfei was constructed by using pulsed-field gel electrophoresis of restriction fragments generated by digestion with NotI and NheI. The chromo- some was found to be circular and 1,729 kb in size. Twenty-eight genes were mapped to specific restriction enzyme fragments by performing hybridization experi- ments with gene probes from various Methanobacterium strains. The genomic map obtained was compared with the updated genomic map of Methanobacterium thermo- autotrophicum Marburg. In spite of major restriction pattern dissimilarities, the overall genetic organization seemed to be conserved between the genomes of the two strains. In addition, the two rRNA operons of strain Mar- burg were precisely mapped on the chromosome, and it was shown that they are transcribed in the same direction.
Databáze:	OpenAIRE
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