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The analysis of cyclocytidine using radioactive tracer techniques coupled with instant thin-layer chromatography depends upon maintaining the stability of the compound throughout the analysis. At pH 7.0 or below cyclocytidine is stable in phosphate, citrate, and formate buffer, but the addition of heavy metal ions to these buffers may yield unstable conditions. The data emphasize the need for carefully controlled conditions in the analysis of cyclocytidine. In general, the data are in agreement with previous reports in that cyclocytidine is hydrolyzed to arabinosylcytosine in alkaline solutions. Based upon the experiments reported herein, we suggest that the hydrolysis of cyclocytidine is non-enzymatic, and that a number of factors related to sample handling and chromatographic conditions will influence the rate of hydrolysis. |
