Colonization of frogRana temporariaL. urinary bladder by Gram-negative bacteria leads to decreased effect of arginine-vasotocin on water reabsorption from the urinary bladder
| Autor: | Rimma G. Parnova, Lavrova Ea, E. M. Fock |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Forskolin Gram-negative bacteria Urinary bladder Lipopolysaccharide biology Physiology Reabsorption biology.organism_classification Enterobacteriaceae Epithelium chemistry.chemical_compound medicine.anatomical_structure Endocrinology chemistry Internal medicine Genetics medicine Osmoregulation Animal Science and Zoology Molecular Biology Ecology Evolution Behavior and Systematics |
| Zdroj: | Journal of Experimental Zoology Part A: Ecological Genetics and Physiology. |
| ISSN: | 1932-5223 |
| Popis: | In frogs and toads the urinary bladder is very important for the maintenance of water balance due to its ability to store water which can be reabsorbed under the action of arginine-vasotocin (AVT). The usage of isolated bladders as a model for studying the osmotic water permeability (OWP) regulation has a disadvantage which relates to high variability of AVT effect among individuals, some showing insensitivity to the hormone. We hypothesized that the response of the bladder to AVT could depend on the colonization of the mucosal epithelium by Gram-negative bacteria. To test this, paired hemibladders of the frog Rana temporaria were used for measurement of OWP and for analysis of Gram-negative bacteria in the bladder tissue or isolated epithelial cells. Among the 206 frogs studied, 41% were infected by different Enterobacteriaceae, with prevalence of Hafnia alvei and Escherichia coli. In infected bladders the basal level of OWP was unchanged, whereas OWP stimulated by AVT was reduced (non-infected: 2.53 ± 0.13, n = 59, infected: 1.21 ± 0.17 µL min−1 cm−2, n = 38, for the 15 min of AVT action, P |
| Databáze: | OpenAIRE |
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