Chaptalia nutans Polak: Root Extract Has High In Vitro Antioxidant Activity and Low Cytotoxicity In Vivo
| Autor: | Vanusa Manfredini, Letiele Bruck de Souza, Jefferson Enderle, Elton Luis Gasparotto Denardin, Thainara de Andrade Fortes, Rafael Roehrs, Amanda Leitão Gindri |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Journal of Medicinal Food. 24:161-171 |
| ISSN: | 1557-7600 1096-620X |
| DOI: | 10.1089/jmf.2019.0309 |
| Popis: | The Asteraceae family is widely known for its therapeutic, aromatic, and nutritional properties. Chaptalia nutans (C. nutans), a member of the family, is widely used in folk medicine in southern Brazil. In this study, we aim to assess compounds present in root extracts of C. nutans, and evaluate their antioxidant capacity and toxicity. To determine the chemical composition of the extract, was performed through Liquid Chromatography coupled with Mass Spectroscopy. Antioxidant capacity, toxicity (Artemia salina biosassay), cytotoxicity, genotoxicity (Allium cepa test), and neurotoxicity (Drosophila melanogaster model) were evaluated. A large number of bioactive phytoconstituents were determined to be present, such as alkaloids, coumarins, flavonoids, terpenes, and especially phenolic compounds, which may explain the antioxidant capacity of the extract. Extracts had the capacity to protect cells from protein and lipid damage, and inhibit the formation of oxygen radicals. The A. salina bioassay revealed that extracts were only slightly toxic. In A. cepa, cells exposed to 1.5 mg/mL extract were protected against chromosomal damage caused by glyphosate, and had mitotic index values that were reduced by 49%. A concentration of 10 mg/mL extract did not kill flies, and when coadministered with paraquat (PQ) (52.5%) produced a mortality rate of only 18.75%. These findings indicated that the extract had the potential to protect against PQ-induced neurotoxicity. Taken together, these data reveal for the first time that the root extract of C. nutans is a rich source of natural antioxidants. The extract may be useful in the food and pharmaceutical industries. |
| Databáze: | OpenAIRE |
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