| Autor: |
C. Lonardo, G. Pastores, B. Variano, M. L. Anderson |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
Journal of Liquid Chromatography. 18:3049-3059 |
| ISSN: |
0148-3919 |
| DOI: |
10.1080/10826079508010432 |
| Popis: |
A rapid, reversed phase, high performance liquid chromatography (HPLC) method for the quantitation of porcine insulin has been developed and validated at Emisphere Technologies. Insulin is assayed with a C4 silica based column in a matrix containing a patented Proteinoid Oral Drug Delivery System (PODDS) for the oral delivery of protein and non-protein drugs. This technology is based on low molecular weight, thermally condensed and acylated amino acids (proteinoids) forming pH dependent microspheres to encapsulate the insulin. Chemical modification of the proteinoids were shown to enhance drug delivery but interfered with the HPLC assay by co-eluting with the insulin. This resulted in distortions to the insulin peak shape reduceing the accuracy and precision of the quantitative data. This method was then re-developed and the interferences removed by employing a unique, non-porous, poly(styrenedivinylbenzene) (PSDVB) packing material. The absence of pores eliminates the column efficiency (N) and resolution (R) values important for the separation of small molecules which conform to the principals of partition chromatography. Consequently the small proteinoid molecules elute at the column void while large proteins such as insulin, which conform to the principals of partition chromatography, elute at a later time. The result is complete resolution of large proteins from peptides and low molecular weight compounds present in the same matrix. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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