PP106

Autor: Hirofumi Tomioka, Hiroyuki Harada, Sho Matsukawa, Kei-ichi Morita, Ken Omura, Pradit Rushatamukayanunt
Rok vydání: 2013
Předmět:
Zdroj: Oral Oncology. 49:S130
ISSN: 1368-8375
DOI: 10.1016/j.oraloncology.2013.03.349
Popis: Background This study aimed to examine the association between human papillomavirus (HPV) and molecular markers in young patients with oral squamous cell carcinoma (SCC). Methods From the cohort of patients enrolled for the treatment of oral SCC in our department during 2001–2009, we identified 40 cases with adequate formaldehyde-fixed paraffin-embedded (FFPE) samples. The median age at diagnosis was 32 years (the range was 21–38 years). Diagnostic procedures were studied on FFPE specimens. The HPV test was performed using a Roche-AMPLICOR® Human Papillomavirus detection kit. Immunohistochemical (IHC) staining was tested for detection of the p16, p53 and Fas-associated death domain containing protein (FADD). The correlation between IHC staining, HPV status, and outcome was analyzed. Results HPV was detected in 2 out of 40 patients (5%), whereas p16 overexpression was observed in 13 cases (32.5%). Overexpressions of p53 and FADD were found in 13 cases (32.5%) and 17 cases (42.5%), respectively. There was negative correlation between the overexpression of p16 and tumour differentiation (Pearson’s correlation r = −0.413; p = 0.008). We also detected positive correlation between the overexpression of p53 and FADD (Pearson’s correlation r = 0.483; p = 0.002). However, there was no marked association between the p16 expression and HPV status. Furthermore, molecular markers were not found to be relevant in assessing clinical outcome. Conclusion The expression of p16 is not an appropriate surrogate marker for HPV infection in oral SCC, and it is unlikely that HPV infection plays an important role in carcinogenesis of oral SCC in young Japanese patients.
Databáze: OpenAIRE
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