| Autor: |
J M Carroll, Jean-Pierre Molès |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
Experimental Dermatology. 9:20-24 |
| ISSN: |
0906-6705 |
| DOI: |
10.1034/j.1600-0625.2000.009001020.x |
| Popis: |
The study of mouse epidermal biology has been hampered by the lack of a good in vitro model for the culture of mouse keratinocytes which allowed the reconstruction of a fully differentiated epidermis. We adapted the Prunieras' model, also called the Dead de-Epidermized Dermis model (DED), to mouse keratinocytes and showed that a neo-epidermis can be reconstructed exhibiting a complete differentiation program. We also used this model to culture transgenic mouse keratinocytes. We observed that transgene expression occurred in the correct location and that the neo-epidermis mimed previous in vivo observations obtained with integrin skin-targeted transgenic mice. Therefore, this model will be a powerful tool to further investigate normal mouse and transgenic keratinocyte biology. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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