Comparison of lisianthus (Eustoma grandiflorum) cultivars based on the selected regeneration media using anther culture

Autor: Wang Jihua, Zhou Xuhong, Ying Luo, Wu Min, Qu Suping, Jinze Li, Mo Xijun, Tian Min, Gui Min, Wu Xuewei
Rok vydání: 2014
Předmět:
Zdroj: Horticulture, Environment, and Biotechnology. 55:125-128
ISSN: 2211-3460
2211-3452
DOI: 10.1007/s13580-014-0013-x
Popis: Double haploids in lisianthus cultivars were produced using anther culture. The anther of ‘Art Peach’ was cultured on the Murashige and Skoog (MS) medium with 3% sucrose with auxin and cytokinin. The highest frequency of calli developed from anthers (100%) and green plantlets per 100 calli (60 regenerants) was observed on the medium containing 8 mg·L−1 N6-benzyladenine (BA) and 0.1 mg·L−1 naphthaleneacetic acids (NAA). When anthers were cultured on the medium supplemented with 2 mg·L−1 BA and 0.5 mg·L−1 NAA, 33.3% of calli formed plantlets. Using the anther culture, callus induction and plantlets regeneration in five cultivars were developed differently, suggesting that the genotype might predetermined anther culture response. These results indicate that a highly efficient new microprogation technique can be provided by anther culture in vitro for lisianthus. Anther culture in vitro can provide a highly efficient new microprogation technique for lisianthus.
Databáze: OpenAIRE
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