Possible role of arachidonic acid in the regulation of lactate production in rat Sertoli cells
| Autor: | Maria Fernanda Riera, Selva B. Cigorraga, Eliana Herminia Pellizzari, Helena Fedora Schteingart, Silvina Beatriz Meroni |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
endocrine system
medicine.medical_specialty biology Urology Endocrinology Diabetes and Metabolism Glucose uptake Glucose transporter Metabolism Sertoli cell chemistry.chemical_compound Phospholipase A2 medicine.anatomical_structure Endocrinology Reproductive Medicine chemistry Internal medicine Lactate dehydrogenase medicine biology.protein lipids (amino acids peptides and proteins) Arachidonic acid Unsaturated fatty acid |
| Zdroj: | International Journal of Andrology. 26:310-317 |
| ISSN: | 0105-6263 |
| DOI: | 10.1046/j.1365-2605.2003.00432.x |
| Popis: | The aim of the study was to determine whether arachidonic acid (AA) is involved in the regulation of Sertoli cell lactate production and if this fatty acid participates in follicle-stimulating hormone (FSH) regulation of Sertoli cell function. In a first set of experiments the effect of AA and porcine pancreas phospholipase A2 (PLA2) on lactate production, glucose uptake, lactate dehydrogenase (LDH) activity and LDH A mRNA levels in Sertoli cell cultures obtained from 20-day-old rats was evaluated. In a second set of experiments the effect of two PLA2 inhibitors--quinacrine (Q) and AACOCF3--on FSH stimulation of the above-mentioned parameters of Sertoli cell function was investigated. Treatment with PLA2 and AA increased Sertoli cell lactate production. The observed action of exogenously added PLA2 involved its catalytic properties responsible for AA release. PLA2 and AA treatments also stimulated Sertoli cell glucose uptake, LDH activity and LDH A mRNA levels. In order to determine whether AA participates in FSH regulation of Sertoli cell lactate production cells were incubated with FSH in the absence or presence of the PLA2 inhibitors Q and AACOCF3. Both drugs partially inhibited the ability of FSH to stimulate lactate production, glucose uptake and LDH activity. The present investigation suggests that AA is involved in the regulation of lactate production, glucose transport, LDH activity and LDH A mRNA levels. In addition, these results suggest that cytosolic PLA2 and AA may participate in FSH-regulation of Sertoli cell energetic metabolism. |
| Databáze: | OpenAIRE |
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