The use of eosin B to assess the viability and developmental potential of rat embryos

Autor: Michael Patrick Dooley
Rok vydání: 2018
Předmět:
DOI: 10.31274/rtd-180813-11124
Popis: x INTRODUCTION 1 Prelmplantatlon Period of Embryonic Development 1 Assessment of Frelmplantation Embryonic Viability 3 Background and Review of Literature 6 Morphological evaluation 6 In vitro development 10 In vivo development 14 Metabolic studies 16 Precursor uptake 16 Metabolic by-product 18 Isotope release 19 Dye assays 20 Dye metabolism 20 Vital staining 21 Nonvital staining 25 Dye-exclusion assays 27 Embryonic and/or maternal factors 28 STATEMENT OF THE RESEARCH PROBLEM 30 RESEARCH OBJECTIVE 31 MATERIALS AND METHODS 33 Animals 33 Strains utilized 33 Selection criteria 34
Databáze: OpenAIRE