The partitioning of protease from Calotropis procera latex by aqueous two-phase systems and its hydrolytic pattern on muscle proteins
| Autor: | Phanuphong Chaiwut, Punyawatt Pintathong, Soottawat Benjakul, Saroat Rawdkuen |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Muscle protein
Protease Aqueous solution Chromatography biology General Chemical Engineering medicine.medical_treatment Polyethylene glycol biology.organism_classification Biochemistry Hydrolysis chemistry.chemical_compound chemistry Calotropis procera Phase (matter) PEG ratio medicine Food Science Biotechnology |
| Zdroj: | Food and Bioproducts Processing. 89:73-80 |
| ISSN: | 0960-3085 |
| DOI: | 10.1016/j.fbp.2010.02.001 |
| Popis: | The protease from the latex of Calotropis procera was isolated by an aqueous two-phase system (ATPS). Polyethylene glycol (PEG 1000, 2000 and 3000) at a concentration of 12, 15, and 18% (w/w) with salts ((NH4)2SO4, K2HPO4 and MgSO4) at a concentration of 14, 17, and 20% (w/w) were investigated. The highest protease recovery (74.6%) was found in the PEG-rich phase of the system (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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