Relation of age to isoenzyme pattern and total activity of amylase in serum

Autor:	P J Bossuyt, Y Van Maercke, S L Scharpé, R Van den Bogaert
Rok vydání:	1981
Předmět:	Chromatography biology Isoelectric focusing Chemistry Biochemistry (medical) Clinical Biochemistry Isozyme Isoenzyme pattern Isoelectric point Biochemistry biology.protein Isoamylase Amylase Polyacrylamide gel electrophoresis Incubation
Zdroj:	Clinical Chemistry. 27:451-454
ISSN:	1530-8561 0009-9147
Popis:	Pancreatic and salivary isoenzymes of amylase were determined in serum from 70 subjects. Thin-layer gel/isoelectric focusing was used to separate the isoenzymes. Because other studies (J. Lab. Clin. Med. 90: 141-151, 1977) show that the major isoamylases have isoelectric points between 5.8 and 7.2, we focused the sera on polyacrylamide gel plates with a pH gradient from 5.5 to 8.5. The separated amylase fractions were made visible by direct incubation with a commercially available dye-starch polymer. Isoelectric focusing proved to be convenient, precise, and reproducible, and it can be used as a routine analysis to detect even slight changes in serum amylase distributions. We found that the isoamylase distribution is age dependent, whereas total amylase activity shows no correlation with age.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::65c8d794cd5b71ecae884b355343debe https://doi.org/10.1093/clinchem/27.3.451 Zobrazit plný text záznamu