Effects of Endothelin and Relaxin on Rat Uterine Segment Contractility1
| Autor: | Peter G. McGovern, Cecilia L. Schmidt, Gerson Weiss, Laura T. Goldsmith, S. Von Hagen, M. Linden |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Relaxin
medicine.medical_specialty medicine.drug_class Uterus Uterine horns Cell Biology General Medicine Peptide hormone Biology Contractility Endocrinology medicine.anatomical_structure Reproductive Medicine Estrogen Internal medicine medicine medicine.symptom Endothelin receptor Muscle contraction |
| Zdroj: | Biology of Reproduction. 46:680-685 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod46.4.680 |
| Popis: | In order to determine the effects of endothelin (ET) and relaxin on uterine contractility, immature female rats were treated with estrogen (E, 1 microgram s.c., Days 1-3) or estrogen and progesterone (2 mg s.c. [E + P], Days 2 and 3), and killed; the uterine horns were removed and suspended in muscle baths. Initially, we determined the contractile response to varying doses of ET and how this response was altered by pretreatment with progesterone. Uterine strips from animals treated with E + P (n = 10) were less sensitive to the stimulatory effects of ET than were strips from E-treated animals (n = 10). This difference was significant at ET doses above 2.5 nM. After completion of the dose-response studies, contractile patterns in response to ET and relaxin were then studied in animals treated with E (n = 10) or E + P (n = 9). ET (5 nM) significantly increased uterine contractility, mostly through an effect on the frequency of contractions (p less than 0.01). Relaxin (25 ng/ml) decreased contractility, affecting all contractile parameters measured (p less than 0.01). ET stimulated contractility in uterine horn segments previously inhibited by relaxin (p less than 0.01), and relaxin reduced the increased contractility produced by earlier exposure to ET (p less than 0.01). These data indicate that ET and relaxin can interact reversibly to control contractility in uterine horn segments in vitro, and that progesterone pretreatment can diminish the contractile response to the stimulatory effects of ET. |
| Databáze: | OpenAIRE |
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